en Research Paper Research Paper <div style="text-align: justify;">Gold nanoparticles (AuNP) were synthesized using edible mushroom <em>Russula delica</em> (RD) in this study. Possibilities to evaluate these synthesized nanoparticles (RD-AuNPs) as bioactive substances were investigated. Characterization of synthesized RD-AuNPswere characterized via UV-vis, XRD, FTIR, EDX. In spherical view, RD-AuNPs with a crystal size of 34.76 nm were synthesized. As a result, fungal systems used for nanomaterial biosynthesis as an effective alternative to chemical synthesis can be used in different biotechnological and medical applications. RD-AuNPs produced by green synthesis can be evaluated in this context.</div> AuNPs,fungus,SEM,UV-Vis,XRD,Antimicrobial 1 8 http://ijmse.iust.ac.ir/browse.php?a_code=A-10-3476-1&slc_lang=en&sid=1 Hilal Acay hilalacay@gmail.com 1800319475328460014732 1800319475328460014732 Yes Department of Nutrition and Dietetic, Faculty of Health Science, Mardin Artuklu University, 47200, Mardin, Ayfer Yıldırım ayferyildirim@artuklu.edu.tr 1800319475328460014733 1800319475328460014733 No Medical Laboratory Techniques, Vocational Higher School of Healthcare Studies, Mardin Artuklu University Ayşe Baran ayse.gorgec43@gmail.com 1800319475328460014734 1800319475328460014734 No Mardin Artuklu University, Graduate Education Institute,